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二手液質聯用儀

二手AB Sciex API 3200液質聯用儀

發布時間:2019/07/15 點擊量:

二手AB Sciex API 3200/3200QTRAP液質聯用儀
 

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簡介:

   API 3200™ LC/MS/MS系統集合了公司的最新質譜技術,其性能可滿足最嚴格法規要求的應用領域,是小分子高通量定量分析實驗室首選的儀器。此儀器可應用于所有定量分析領域如環境分析、食品安全分析、臨床研究和藥物研究領域,其數據的可靠性和重現性非常好;如行業領先的API 5000™和API 4000™系統一樣,API 3200™也能提供高質量的分析數據,它的硬件包括:

  • 創新的Turbo V™離子源—即插即用式探頭,離子化效率高,定量分析時靈敏度高,適應寬范圍的液相流速

  • LINAC®碰撞室技術—快速掃描能力

  • 專利氣簾接口—降低交叉污染,提高離子化效率

  • 高效率的軟件應用—包括自動方法開發

  • 高級采集功能和Analyst®軟件處理數據功能—方法自動建立和分析

  • 可選的DuoSpray™離子源—快速方法開發和增加分析通量

  • 可選的PhotoSpray® 離子源—擴展了儀器的應用領域

  • 可選的NanoSpray®離子源 — 提高了低流速條件下的分析靈敏度

    Principles of the System
    This section describes features and applications of the API 3200™ LC/MS/MS
    system.
    Applications for the System
    The API 3200 system is designed for quantitative analysis of small molecules.This application involves measurement of specific molecular weight compounds, usually a drug or metabolite in a liquid sample, and their resulting fragment ions for determining the exact quantity of the compound in the sample of interest. Quantitation is performed using a standard curve of mass spectrometer signal intensity for various known concentrations of the compound. The signal in the test sample is then compared to the standard curve to determine the concentration.The typical scan type used for this application is MRM (Multiple Reaction Monitoring).
     
    Features of the Instrument
    The API 3200 system is a benchtop triple quadrupole mass spectrometer for sensitive quantitative analyses of biomolecules found in a wide variety ofbiological matrices. It has the following features:
    • The standard Turbo V™ ion source provides a plug-and-play TurboIonSpray® probe and APCI probe, a durable ceramic interface, and compatibility with high flow rates.
    • LINAC® collision cell eliminates cross-talk and allows for multi-component analysis during an LC run.
    • Rugged Curtain Gas™ interface technology combined with improved gas dynamics reduces maintenance and increases uptime.
    • Scanning in a mass range of m/z 5–1800.
    • Integrated switching valve and integrated syringe pump provide convenient sample introduction flexibility.
    • Additional compatible sources, including the DuoSpray™ ion source, PhotoSpray® ion source, and NanoSpray® III ion source components, provide further flexibility.
    • IDA (Information Dependent Acquisition) maximizes the amount of information that can be acquired in an LC scan.
    • Compatible with additional software packages, such as Metabolite ID software, Automaton software, BioAnalyst™ software, and more.
     
    How the Instrument Works
    The API 3200 mass spectrometer has a series of quadrupole filters that transmit ions according to their m/z (mass-to-charge ratios). Ions entering the ion path are first focused by Q0 before passing into Q1.Q1 is a filtering quadrupole that sorts the ions before they enter Q2, a LINACcollision cell in which ions can be broken into fragments by collisions with gas molecules. This technique allows for the design of experiments that measure the m/z of product ions to determine the composition of the parent ions. After passing through Q2 the ions enter Q3 for additional filtering, and then enter the detector. In the detector, the ions create a current that is converted into a voltage pulse. The voltage pulses leaving the detector are directly proportional to the quantity of ions entering the detector. The instrument monitors these voltage pulses and converts the information into a signal. The signal represents the ion intensity for a particular m/z and the instrument displays this formation as a mass spectrum.


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